NaNO3 ........................2.0 g
MgSO4 ........................0.5 g
KCl..........................0.5 g
Fe2(SO4)3 . H2O ...............0.01 g
KH2PO4 .......................0.14 g
K2HPO4 .......................1.2 g
Agar........................15.0 g
Yeast extract................0.02 g
Distilled water..............1.0 L
Autoclave at 121C for 15 minutes.
Adjust pH to 7.2. Place strip of sterile filter paper on cooled agar slant and inoculate on filter strip.