Malic acid.........................2.5 g
Yeast Extract (BD 212750)..........1.0 g
(NH4)2SO4...........................1.25 g
MgSO4 . 7H2O .......................0.2 g
CaCl2 . 2H2O .......................0.07 g
Ferric citrate.....................0.01 g
EDTA...............................0.02 g
KH2PO4 .............................0.6 g
K2HPO4 .............................0.9 g
Trace Elements (see below).........1.0 ml
Vitamin Solution (see below).......7.5 ml
Distilled water to.................1.0 L
Neutralize malic acid with NaOH and adjust the pH of the completed medium to 6.9.
Autoclave at 121C for 15 minutes.
Trace Elements:
Ferric citrate.....................0.3 g
MnSO4 . H2O ........................0.002 g
H3BO3 ..............................0.001 g
CuSO4 . 5H2O .......................0.001 g
(NH4)6Mo7O24 . 4H2O..................0.002 g
ZnSO4 ..............................0.001 g
EDTA...............................0.05 g
CaCl2 . 2H2O .......................0.02 g
Distilled water..................100.0 ml
Vitamin Solution:
Nicotinic acid.....................0.2 g
Nicotinamide.......................0.2 g
Thiamine . HCl.....................0.4 g
Biotin.............................0.008 g
Distilled water....................1.0 L