Component I
MgSO4………………………………2.0 g
Sodium Citrate……………………..5.0 g
CaSO4 x 2H2O……………………..1.0 g
NH4Cl……………………….………1.0 g
DI Water…………………………….400.0 ml
Component II
K2HPO4……………………………..0.5 g
DI Water…………………………….200.0 ml
Component III
Sodium Lactate….…………………3.5 g
Yeast Extract………………………..1.0 g
DI Water……………………………..400.0 ml
Each component is adjusted to pH 7.5 and autoclaved at 121?C. The three components are mixed aseptically and tubed under 97% N2 and 3% H2. Tube while warm to exclude as much oxygen as possible.
Sterilize by Filtration
5% Ferrous Ammonium Sulfate, Fe(NH4)2(SO4)2; Biologist will add 0.1 ml to 5.0 ml of medium prior to inoculation.
*****Note: Ferrous Ammonium Sulfate solution is optional and should not be added unless specified.