CaCO3 .............................................0.2 g
V8 Juice...........................................50.0 ml
Agar.................................................20.0 g
Rye broth (see below).....................1000 ml
Autoclave at 121?C.
Rye broth
Whole rye grains...............................50.0 g
DI Water...........................................1100 ml
Soak grains in distilled water for 24-36 hours at 24?C.
Autoclave at 121?C for 30 minutes. Filter through 4 layers of cheesecloth and adjust the final filtrate volume to 1000 ml.