Heart Infusion Broth (BD 238400).......................................7.5 g
Agar, Noble (BD 214230) (if necessary).............................10.0 g
DI Water…….......................................................................660.0 ml
Adjust for final pH 7.4 and autoclave at 121?C.
Add aseptically the following filter-sterilized components:
10X Hanks' Balanced Salt Solution w/o NaHCO3 or phenol red (see below) ....................................40.0 ml
0.25% Phenol red................................................................10.0 ml
Porcine serum (heat-inactivated).........................................200.0 ml
5 % Lactalbumin Hydrolysate, TC (BD 259962) in 1 X Dulbecco's PBS (GIBCO 14040)...............................100.0 ml
Yeast Extract Solution (GIBCO 18180)................................20.0 ml
Hanks' Balanced Salt Solution (10X) CaCl2 ..........................................1.4 g
KCl...............................................4.0 g
KH2PO4 ......................................0.6 g
MgCl2 x 6H2O .............................1.0 g
MgSO4 x 7H2O ...........................1.0 g
NaCl..............................................80.0 g
Na2HPO4 x 7H2O........................0.9 g
Glucose........................................10.0 g
DI Water to...................................1000.0 ml