Nutrient Broth (BD 234000).........8.0 g
Yeast extract......................2.0 g
K2HPO4 .............................2.0 g
KH2PO4 .............................0.5 g
Glucose Solution (see below).......5.0 g
MgSO4 . 7H2O (see below) ...........0.25 g
Agar..............................15.0 g
Distilled water..................940.0 ml
Autoclave for 25 minutes at 121C.
Glucose Solution:
Prepare a 10% (w/v) solution of glucose, filter-sterilize and aseptically add 50.0 ml to sterile basal medium.
Magnesium Sulfate Solution:
Prepare a 2.5% sterile solution of MgSO4 . 7H2O and aseptically add 10.0 ml to the sterile basal medium.