NaNO3……………………....................................1.5 g
K2HPO4…………………......................................0.04 g
MgSO4 x 7H2O………………..............................0.075 g
CaCl2 x 2H2O………………................................0.036 g
Citric acid.............................................................6.0 mg
Ferric ammonium citrate......................................6.0 mg
EDTA...................................................................1.0 mg
Na2CO3 ..............................................................0.02 g
Trace Metal Mix A5 (see below)..........................1.0 ml
Agar (if needed)...................................................12.0 g
DI Water…………………………………………….1000 ml
Adjust final pH to 7.1.
Autoclave at 121?C.
Trace Metal Mix A5 H3BO3…………………….....................................2.86 g
MnCl2 x 4H2O………………….............................1.81 g
ZnSO4 x 7H2O…………………............................0.222 g
Na2MoO4 x 2H2O………………...........................0.39 g
CuSO4 x 5H2O………………................................0.079 g
Co(NO3)2 x 6H2O………………...........................49.4 mg
DI Water……………………………………………..1000 ml