ATCC Medium: 2789 Artificial Mono Lake Medium
(NH4)2SO4 .................................................................... 0.1g
MgSO4.7H2O………………………………………………0.025g
NaCl……………………………………………………….60g
KH2PO4……………………………………………………0.08g
K 2PO4………………………………………………………0.150g
Na 2CO3 …………………………………………………….10.6g
NaHCO3 ……………………………………………………4.2g
SL10 Trace Elements (see below)………………………5 ml
Glucose solution (add aseptically; see below)…………50 ml
Wolfe’s Vitamin (add aseptically)…………………….…10 ml
DI Water……………………………………………………940 ml
Mix all ingredients except for vitamins and glucose solution. Adjust pH to 9.85 with NaOH. Autoclave at 121?C and let cool. Aseptically add vitamins and glucose solution. Dispense into aerobic culture vessels as desired.
SL10 Trace Elements
HCl (25%)…………………………………………………..10.0 ml
FeCl2 x 4H2O………………………………………………1.50 g
ZnCl2………………………………………………………..70.0 mg
MnCl2 x 4H2O………………………………………………100.0 mg
H3BO3……………………………………………………….6.0 mg
CoCl2 x 6H2O………………………………………………190.0 mg
CuCl2 x 2H2O………………………………………………2.0 mg
NiCl2 x 6H2O……………………………………………….24.0 mg
Na2MoO4 x 2H2O…………………………………………36.0 mg
Na2WO4 x 2H2O………………………………………….3.0 mg
DI Water…………………………………………………….990.0 ml
Glucose……………………………………………………1.8 g
Glucose Solution
DI Water……………………………………………………50 ml