ATCC medium: 665 YTN medium
Yeast extract.....................10.0 g
Tryptone (BD 211705)..............10.0 g
NaCl..............................30.0 g
Glucose............................1.0 g
Trace Elements (see below).........1.0 ml
Agar..............................15.0 g
Tap water..........................1.0 L
Adjust pH to 7.2. Autoclave at 121C for 15 minutes.
Trace Elements:
H3BO3 ..............................2.85 g
MnCl2 . 4H2O .......................1.8 g
FeSO4 ..............................1.36 g
Sodium tartrate....................1.77 g
CuCl2 . 2H2O ......................26.9 mg
ZnCl2 .............................20.8 mg
CoCl2 . 6H2O ......................40.4 mg
Na2MoO4 . 2H2O.....................25.2 mg
Distilled water....................1.0 L