ATCC Medium: 1271 Benzoate Medium
(NH4)2HPO4................................................3.0 g
KH2PO4…………….....................................1.2 g
NaCl..............................................................5.0 g
MgSO4 x 7H2O…………..............................0.2 g
Yeast Extract……………..............................0.5 g
Agar, Noble (BD 214230).............................20.0 g
DI Water……………………………………….950 ml
Combine all ingredients except sodium benzoate. Autoclave at 121?C. Aseptically add the following filter-sterilized sodium benzoate solution:
Sodium Benzoate
Solution Sodium benzoate……………………………..3.0 g
DI Water………………………………………..50 ml