【编号】 0171
【名称】 牛肝浸液培养基
【成份】 Diced beef liver 500.0 g
Peptone 10.0 g
K2HPO4 1.0 g
Water 1.0 L
[note]:
(1)Putting 500g of the diced and de-fat beef liver in 1.0 L water
immersed at refrigerator over night
autoclave for 10 min.
filtration by suction.
(2)Adding 10g of peptone and 1g of K2HPO4
adjusting pH to 8.0
filtrating with paper
making the volume into 1.0 L.
(3)The beef liver residue retained.
(4)Distributing the broth into tube up to 1 in. then adding the beef liver residue in tube up to 2 in.(total)
and CaCO3 in trace.