ATCC Medium: 28 Sabouraud Dextrose Agar, Emmons Modification
Agar Medium
Sabouraud Agar Modified (BD 274720)…………..50.0 g
DI Water……………………………………………...1000 ml
Autoclave at 121? C.
Broth Medium Sabouraud Broth (BD cat 238230)………………..30.0g
DI Water…………………………………….…….….1000 ml
Autoclave at 121? C.
*This product is commercially available from BD.
To make medium from scratch, follow formulation below:
Sabouraud Dextrose Agar, Emmons Modification Composition
Neopeptone……………………………..…………10.0 g
Dextrose…………………….……………..……….20.0 g
Agar……………………………………....………...20.0 g
DI Water……………………....................…………1000 ml
Final pH 7.0 +/- 0.2.
Omit agar for broth medium.
*This medium is a modification of regular Sabouraud Dextrose Agar.
It contains a reduced amount of dextrose and has a neutral pH.