ATCC Medium: 173 Middlebrook 7H10 with OADC
Middlebrook 7H10………………………………………….….19.0 g
Glycerol…………………………………………………….…...5.0 ml
Dubos Oleic Albumin Complex (OADC) (see below)……...100.0 ml
DI Water………………………………………………….……..900.0 m
l Add and mix all components except for OADC. Bring to a boil to dissolve agar and autoclave at 121?C. Cool to 50-55?C and aseptically
OADC.
OADC Oleic Acid (Sigma Cat # O-1008)……………………………..0.05 g
Albumin Fraction V Bovine (Sigma A9647)……….………...5 g
Dextrose (BD 215530)…………………………………………2 g
Catalase (beef) (Sigma C-9322)……………………………...0.004 g
NaCl……………………………………………………………..0.85 g
DI Water…………………………………………………………100 ml
OADC is available from BD (237510) or can be made following the formulation above.