拟杆菌纲的种类很多生活在人或者动物的肠道中,有些时候成为病原菌。在粪便中,以细胞数目计,拟杆菌属(Bacteroides)是主要微生物种类。
一、菌种简介
平台编号:Bio-79895
规格:Freeze-Dried
拉丁属名:Bacteroides Stercoris
菌株名称:粪便拟杆菌
用途:ATCC原装进口
注意事项:仅用于科学研究或者工业应用等非医疗目的不可用于人类或动物的临床诊断或治疗,非药用,非食用(产品信息以出库为准)
二、详细描述
Bacteroides stercoris Johnson et al. 拉丁名
(ATCC? 43183?) 统一编号
Strain Designations 菌种别名 : VPI B5-21 [CIP 104203T, JCM 9496, subsp. a] /
Type Strain 模式菌种: yes /
Biosafety Level 生物安全等级 : 2
Application 用途 Characterization Isolation Human feces, Texas
Biosafety Level 生物安全等级 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format 提供形式 freeze-dried
Storage Conditions 保藏方法
Frozen(冷冻物): -80℃ or colder
Freeze-Dried(冻干物): 2℃ to 8℃
Live Culture(活物): See Propagation Section
Preceptrol? no Genome Sequenced Strain Yes
Type Strain 模式菌种 yes
Comments 注释 This strain has been sequenced as a reference genome for the NIH Human Microbiome Project.
Medium 培养基 ATCC? Medium 1490: Modified chopped meat medium
Growth Conditions 生长条件
Temperature 培养温度: 37℃
Atmosphere 需氧情况 : Anaerobic gas mixture, 80% N2-10% CO2-10% H2
Name of Depositor 寄存人 LV Moore
Chain of Custody 来源国家 ATCC <-- LV Moore <-- W.E.C. Moore
Isolation 分离基物 Human feces, Texas
References 参考文献 Johnson JL. Bacteroides caccae sp. nov., Bacteroides merdae sp. nov., and Bacteroides stercoris sp. nov. isolated from human feces. Int. J. Syst. Bacteriol. 36: 499-501, 1986.
Johnson JL. Taxonomy of Bacteroides I. Deoxyribonucleic acid homologies among Bacteroides fragilis and other saccharolytic Bacteroides species. Int. J. Syst. Bacteriol. 28: 245-256, 1978.
Johnson JJ, Ault DA. Taxonomy of the bacteroides II. Correlation of phenotypic characteristics with deoxyribonucleic acid homology groupings for Bacteroides fragilis and other saccharolytic Bacteroides species. Int. J. Syst. Bacteriol. 28: 257-268, 1978.
Cross References
Nucleotide (GenBank) : X83953 B.stercoris 16S rRNA (ATCC 43183T).
Nucleotide (GenBank) : U50417 Bacteroides stercoris 16S ribosomal RNA gene, partial sequence.
Nucleotide (GenBank) : ABFZ02000000 Bacteroides stercoris ATCC 43183, whole genome shotgun sequencing project。
三、菌株形态
菌落直径为1-2mm,圆形,边缘整齐,不透明,正面白色,中间凸起,表面光滑,表面明亮,质地湿润,易挑起,G-(红),杆菌。
四、传代方法
①准备上述平板 1-2 块;
②平板表面消毒后在安全柜中打开;
③吸取 1-3ml 无菌水(置于厌氧环境平衡24h)打入平板中,用无菌涂布棒在平板表层来回刮取菌苔,制成菌悬液;
④用巴氏吸管吸取菌悬液,打入到新鲜的平板上,涂布均匀;
⑤将平板置于上述培养条件下培养即可。
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