菌种简介
平台编号：Bio-50864
规格：Frozen
拉丁属名：Chlamydia Trachomatis Serovar Ba
菌株名称：沙眼衣原体血清型BA
用途：ATCC原装进口
注意事项：仅用于科学研究或者工业应用等非医疗目的不可用于人类或动物的临床诊断或治疗，非药用，非食用（产品信息以出库为准）
详细信息
Chlamydia trachomatis Serovar Ba 拉丁名
(ATCC® VR-347™) 统一编号
Classification Chlamydiaceae, Chlamydia Deposited As Trachoma serotype ba Agent Chlamydia trachomatis Serovar Ba Strain Apache-2
Applications 用途 Sexually Transmitted Disease Research
Biosafety Level 生物安全等级 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format frozen 1 mL per vial
Storage Conditions 保藏方法 -70°C or colder
Chlamydia trachomatis ATCC VR-347
Comments 注释 FBS used to culture Chlamydia must be prescreened to verify that the serum does not contain antibodies to Chlamydia or other factors that would interfere with growth.
Please see ATCC Virology Guide for more detailed information on propagation.
Effect on Host Intracellular inclusion bodies visualized by fluorescent staining
Recommended Host McCoy cells (ATCC CRL-1696)
Growth Conditions 生长条件
Temperature 培养温度 : 36°C
Recommendations for Infection: For best results, infection should be performed on a 80-100% confluent, 24-48 hour old cellular monolayer.
Sonicate seed material for 20 seconds at approximately 240W to disrupt cells.
Prepare dilution of chlamydia in minimum amount of volume (e.g. 1 mL per 25 cm2).
Remove cell growth medium and inoculate with disrupted material. For adsorption, centrifuge at 1500 x g at 25°C for 1 hour. End adsorption by adding agent growth medium.
Incubation: Incubate infected culture for 72 hours at 36°C in a humidified 5% CO2 atmosphere.
Preservation: Controlled rate freeze.
Effect on Host Intracellular inclusion bodies visualized by fluorescent staining
Name of Depositor 寄存人 E Jawetz
Special Collection ATCC Source
Eye of an Apache Indian child from San Carlos, Arizona
References 参考文献 Hanna L, et al. Trachoma viruses isolated in United States. I. Growth in embryonated eggs. Proc. Soc. Exp. Biol. Med. 104: 142-145, 1960. PubMed: 14399529
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