ATCC 16404 黑曲霉 百欧博伟生物
小杨 / 2021-11-22 08:53:06
黑曲霉，半知菌亚门，丝孢纲，丝孢目，丛梗孢科，曲霉属真菌中的一个常见种。广泛分布于世界各地的粮食、植物性产品和土壤中。是重要的发酵工业菌种，可生产淀粉酶、酸性蛋白酶、纤维素酶、果胶酶、葡萄糖氧化酶、柠檬酸、葡糖酸和没食子酸等。有的菌株还可将羟基孕甾酮转化为雄烯。生长适温28℃左右，最低相对湿度为88%，能引致水分较高的粮食霉变和其他工业器材霉变。
菌种简介
平台编号：Bio-67649
规格：Freeze-Dried
拉丁属名：Aspergillus Brasiliensis
菌株名称：黑曲霉
用途：ATCC原装进口
注意事项：仅用于科学研究或者工业应用等非医疗目的不可用于人类或动物的临床诊断或治疗，非药用，非食用
详细信息
Aspergillus brasiliensis Varga et al.拉丁名
(ATCC? 16404?) 统一编号
Deposited As Aspergillus niger van Tieghem
Strain Designations 菌种别名 WLRI 034(120) [CBS 733.88, DSM 1387, DSM 1988, IFO 9455, IMI 149007, NCPF 2275]
Application 用途 Assay of antimicrobial preservatives； Media testing； Preparatory test control； Quality control strain； Sterility testing； Testing fungicides； Transformation host； Validation control；
Reference strain for performance testing culture media listed by the ISO TC 34 SC 9 Joint Working Group 5 in the ISO 11133 and by the Working Party on Culture Media of the International Committee on Food Microbiology and Hygiene (ICFMH-WPCM) Food testing Pharmaceutical and Personal Care
Biosafety Level 生物安全等级 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format 提供形式 freeze-dried
Storage Conditions 生长条件
Frozen 冷冻物: -80°C or colder
Freeze-Dried 冻干物: 2°C to 8°C
Live Culture: See Propagation Section
Type Strain 模式菌株 no
Preceptrol? no
Comments 注释 This strain is recommended by ATCC for use in the tests described in ASTM Standard Test Method E979-91 where only the taxon is specified. This strain was recently re-named as Aspergillus brasiliensis. Use of impedance for preservative efficacy testing Morphology Colonies initially white or yellowish, mycelium growing rapidly producing a dense layer of erect smooth-stiped, conidiophores terminated by globose vesicles bearing phialides (uniseriate) or metulae with phialides (biseriate) which produce dry chains of conidia. Reverse pale to grayish or greenish yellow. Vesicles radiate, initially pale, becoming dark brown to black. Conidia spherical, mid-to-dark brown, highly roughened with ridges and blunt or pointed protuberances, (3-)4-5(-6) μm in diameter. Sporulation may be inhibited when grown in vessels with reduced gas exchange. Colonies may exhibit sectoring with areas of varying levels of sporulation. Use of freshly produced spores as inoculum should reduce sectoring.
Medium 培养基 ATCC? Medium 336: Potato dextrose agar (PDA)
ATCC? Medium 325: Malt extract agar (Blakeslee's formula)
ATCC? Medium 28: Emmons' modification of Sabouraud's agar
Growth Conditions 生长条件
Temperature 培养温度: 20°C to 25°C
Atmosphere 需氧情况: Typical aerobic
Sequenced Data 18S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and 28S ribosomal RNA gene, partial sequence.
TTAAGCATATCAATAA D1D2 region of the 28S Ribosomal RNA gene
Morphology Colonies initially white or yellowish, mycelium growing rapidly producing a dense layer of erect smooth-stiped, conidiophores terminated by globose vesicles bearing phialides (uniseriate) or metulae with phialides (biseriate) which produce dry chains of conidia. Reverse pale to grayish or greenish yellow. Vesicles radiate, initially pale, becoming dark brown to black. Conidia spherical, mid-to-dark brown, highly roughened with ridges and blunt or pointed protuberances, (3-)4-5(-6) μm in diameter. Sporulation may be inhibited when grown in vessels with reduced gas exchange. Colonies may exhibit sectoring with areas of varying levels of sporulation. Use of freshly produced spores as inoculum should reduce sectoring.
生态分布
黑曲霉，广泛分布于土壤、空气和谷物上，可引起食物、谷物和果蔬的霉腐变质，有的可产生致癌性的黄曲霉毒素。
经济用途
黑曲霉是制酱、酿酒、制醋的主要菌种。是生产酶制剂（蛋白酶、淀粉酶、果胶酶）的菌种。 生产有机酸（如柠檬酸、葡萄糖酸等）。农业上用作生产糖化饲料的菌种。可用来测定锰、铜、钼、锌等微量元素和作为霉腐试验菌。干酪成熟中污染会使干酪表面变黑、变质，对奶油也会产生变色。