海分枝杆菌是研究结核分枝杆菌的一种优良模型，其毒力小、传染性低，只需要在二级生物实验室内进行研究（结核分枝杆菌需要三级），并且其与结核分枝杆菌的基因相似性高达95%以上，感染实验可以在斑马鱼上进行，操作方便。
菌种简介
平台编号：Bio-08693
规格： Freeze-Dried
拉丁属名：Mycobacterium Marinum
菌株名称：海分枝杆菌
用途：ATCC原装进口
注意事项：仅用于科学研究或者工业应用等非医疗目的不可用于人类或动物的临床诊断或治疗，非药用，非食用
详细信息
Mycobacterium marinum Aronson 拉丁名
(ATCC? BAA-535?) 统一编号
Strain Designations 别名 M [Moffett Hospital #975973]
Application 用途 Closely related to members of the M. Tuberculosis complex, this pathogenic mycobacterial species can serve as a model for mycobacterial pathogenesis.
Isolation 分离基物 Tissue samples of human lesions, approximately 1992, Clinical Lab, Moffett Hospital, University of California, San Francisco
Biosafety Level 生物安全等级 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format 提供形式 freeze-dried
Preceptrol? no
Type Strain 模式菌种 no
Comments 注释 This strain causes chronic granulomatous, nonlethal disease in the Leopard Frog, Rana pipiens
Genome sequenced strain
Causative agent of swimming pool granuloma or aquarium tank granuloma
Medium 培养基 ATCC? Medium 173: Middlebrook 7H10 agar with Middlebrook OADC enrichment
Growth Conditions 生长条件
Temperature 培养温度: 30.0°C
Atmosphere 需氧情况: Aerobic
Name of Depositor 寄存者 L Ramakrishnan
Special Collection NCRR Contract
Isolation 分离基物 Tissue samples of human lesions, approximately 1992, Clinical Lab, Moffett Hospital, University of California, San Francisco
Year of Origin 收藏年份 1992
References 参考文献 Ramakrishnan L, Falkow S. Mycobacterium marinum persists in cultured mammalian cells in a temperature-restricted fashion.. Infect. Immun. 62: 3222-3229, 1994. PubMed: 8039892
Lalita Ramakrishnan, personal communication
Ramakrishnan L, et al. Mycobacterium marinum causes both long-term subclinical infection and acute disease in the leopard frog (Rana pipiens). Infect. Immun. 65: 767-773, 1997. PubMed: 9009340
Stinear TP, et al. Insights from the complete genome sequence of Mycobacterium marinum on the evolution of Mycobacterium tuberculosis. Genome Res. 18(5): 729-741, 2008. PubMed: 18403782
Cross References
Nucleotide (GenBank) : AF201682 Mycobacterium marinum phenylalanyl-tRNA synthetase chain B (pheTB) gene, partial cds; and PE-PGRS homolog MAG24-1 (mag24-1), E-PGRS homolog MAG24-2 (mag24-2), and PE-PGRS homolog MAG24-3 (mag24-3) genes, complete cds.
Nucleotide (GenBank) : CP000854 Mycobacterium marinum M, complete genome
Nucleotide (GenBank) : CP000895 Mycobacterium marinum M plasmid pMM23, complete sequence
菌种特性
海分枝杆菌，细胞圆球状,在直角两个平面交替分裂形成四联状,一般细胞成对生,单生者罕见,不成链状排列。革兰氏阳性,不运动,兼性厌氧。在MRS培养基上菌落小,呈白色。沿洋菜穿刺线的生长物呈丝状。接触酶阴性,不产细胞色素。功用产酸,菌群,维持肠道微生态平衡。在动物体内对病原微生物有颉颃作用,可竞争性地抑制病原微生物,,产生有益的代谢产物,激活酸性蛋白酶的活性,参与机体的,防止有害物质产生。
使用方法
直接加入配合饲料中或先按一定倍数稀释后,再加入饲料中混匀。也可预先活化后再发酵饲料或直接供动物饮用。具体用量参考产品使用说明。
注意事项
产品仅用于科研实验储存时,提高水分含量则菌群活力。高温制粒时,必须对产品进行包被处理。不饱和脂肪酸对乳酸片球菌具有颉颃作用,配合日粮中添加乳酸片球菌,应尽量避免与添加的油脂直接接触。饲料中的钙也会明显影响乳酸片球菌的活性。