ATCC菌种 CBS 6330 柱状假丝酵母
小杨 / 2019-09-30 09:19:55
柱状假丝酵母(Candida cylindracea)又称为褶假丝酵母(Candida rugosa)，是一种 半子囊无孢子、假丝状、单细胞、非致病的酵母菌。该酵母通常被认为是对人类和其它生命形式都是安全的。
基本信息
平台编号：bio-105178
规格：freeze-dried
拉丁属名：Candida cylindracea
中文名：CBS 6330 柱状假丝酵母
外文名： Candida cylindracea
别 名： 褶假丝酵母
特 征： 半子囊无孢子、假丝状、单细胞
致病性： 非治病
应 用： 生产脂肪酶
详情介绍
Candida cylindracea Yamada et Machida ex Meyer et Yarrow 拉丁名
ATCC 14830 编号
Strain Designations 菌种别名 MS-5 [CBS 6330, CCRC 21431, DSM 2031, NRRL Y-17506]
Application 用途
Produces cholesterol esterase
Produces lipase
Produces Romano cheese flavor
Biosafety Level 安全等级 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format 提供形式 freeze-dried
Storage Conditions 培养条件
Frozen 冷冻物 : -80°C or colder
Freeze-Dried 冻干物 : 2°C to 8°C
Live Culture: See Propagation Section
Type Strain 模式菌株 yes
Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Preceptrol no
Comments 注释
Molecular modeling of lipases
Oleic acid as inducer of lipase production
Effect of oleic acid concentration on lipase production
Cloning of lipase genes
Medium 培养基 ATCC Medium 28: Emmons' modification of Sabouraud's agar
ATCC Medium 200: YM agar or YM broth
ATCC Medium 324: Malt extract agar
Growth Conditions 生长条件
Temperature 培养温度 : 24°C to 26°C
Atmosphere 需氧情况 : Typical aerobic
Sequenced Data
18S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and 26S ribosomal RNA gene, partial sequence
D1D2 region of the 26S ribosomal RNA gene
Name of Depositor 寄存者 Meito Sangyo Co., Ltd.
U.S. Patent Number
3,189,529
Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Isolation 分离基物 Soil
Cross References
Nucleotide (GenBank) : X64703 LIP1 gene for lipase
Nucleotide (GenBank) : X64704 LIP2 gene for lipase
Nucleotide (GenBank) : X66006 S55937, LIP3 gene for lipase
Nucleotide (GenBank) : X66007 S55939, LIP4 gene for lipase
Nucleotide (GenBank) : X66008 S55942, LIP5 gene for lipase
Nucleotide (GenBank) : AF025307 nucleotide sequence of gpd gene
Nucleotide (GenBank) : U45823 26S ribosomal RNA gene, partial sequence
Nucleotide (GenBank) : X16712 mRNA for lipase I, partial coding sequence
Nucleotide (GenBank) : AJ279020 Candida rugosa leu2 gene, oat gene (partial) and gene for SPL1 tRNA
References 参考文献
Yamada K, Machida H. High-activity lipase and method of preparation thereof. US Patent 3,189,529 dated Jun 15 1965
Beaucamp K, et al. Method for the determination of cholesterol. US Patent 3,925,164 dated Dec 9 1975
Lotti M, et al. Cloning and analysis of Candida cylindracea lipase sequences. Gene 124: 45-55, 1993. PubMed: 8440480
Gordillo MA, et al. Stability studies and effect of the initial oleic acid concentration on lipase production by Candida rugosa. Appl. Microbiol. Biotechnol. 43: 38-41, 1995. PubMed: 7766134
Poch M, et al. On-line monitoring of lipase production in fermentation processes. Biotechnol. Tech. 5: 251-254, 1991.
Valero F, et al. Fermentation behaviour of lipase production by Candida rugosa growing on different mixtures of glucose and olive oil. J. Ferment. Bioeng. 72: 399-401, 1991.
Shi H, et al. Production of a Romano cheese flavor by enzymic modification of butter fat. ACS Symp. Series 317: 370-378, 1986.
Meded. Fac. Landbouwwet. Univ. Gent. 57: 2045-2052, 1992.
Meyer SA, Yarrow D. Validation of the names of three Candida species. Mycotaxon 66: 99-101, 1998.
Jaaskelainen S, et al. Production, characterization, and molecular modeling of lipases for esterification. Ann. N.Y. Acad. Sci. 799: 129-138, 1996. PubMed: 8958083
Sanchez A, et al. On-line deterination of the total lipolytic activity in a four-phase system using a lipase adsorption law. J. Biosci. Bioeng. 87: 500-506, 1999.
Lee GC, et al. Analysis of the gene family encoding lipases in Candida rugosa by competitive reverse transcription-PCR. Appl. Environ. Microbiol. 65: 3888-3895, 1999. PubMed: 10473391
Kurtzman CP, Robnett CJ. Identification of clinically important ascomycetous yeasts based on nucleotide divergence in the 5' end of the large-subunit (26S) ribosomal DNA gene. J Clin Microbiol 35: 1216-1223, 1997. PubMed: 9114410
Longhi S, et al. Cloning and nucleotide sequences of two lipase genes from Candida cylindracea. Biochim. Biophys. Acta 1131: 227-232, 1992. PubMed: 1610906
Kawaguchi Y, et al. The codon CUG is read as serine in an asporogenic yeast Candida cylindracea. Nature 341: 164-166, 1989. PubMed: 2506450
Valero F, et al. Lipase production of Candida rugosa: fermentation behaviour. Biotechnol. Lett. 10: 741-744, 1988.
Obradors N, et al. Effects of different fatty acids in lipase production by Candida rugosa. Biotechnol. Lett. 15: 357-360, 1993.
Kurtzman CP, Robnett CJ. Systematics of methanol assimilating yeasts and neighboring taxa from multigene sequence analysis and the proposal of Peterozyma gen. nov., a new member of the Saccharomycetales. FEMS Yeast Res 10: 353-361, 2010. PubMed: 20522116
Xu L, et al. Cloning of a novel lipase gene, lipJ08, from Candida rugosa and expression in Pichia pastoris by codon optimization. Biotechnol Lett. 32: 269-276, 2010. PubMed: 19841868
柱状假丝酵母(C. cylindracea)，是一种半子囊不产孢子的假丝状单细胞非致病酵母真菌。该酵母通常被认为是对人类和其它生命形式都是安全的。其所产的脂肪酶（C. rugosa lipase , CRL）广泛应用于传统与现代工业，如脂肪酸的生产、各种酯类的合成等，是当前世界上应用最广泛的商品化脂肪酶之一。
另外，在巧克力的生产过程中，柱状假丝酵母起到了很大的作用。可可树的种子内含有大量的糖，非常适合柱状假丝酵母的生长。它能将种子内的这些糖转化为乙醇，并分解果胶质。如果没有柱状假丝酵母的参与，最终的产品是非常苦涩的。最终，柱状假丝酵母会由于高浓度的醇而死亡，可可豆经过一些后续化处理如干燥，包装得到最终的产品。