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生长状态：贴壁生长
ATCC Number：TIB-71
相关疾病：其他疾病
运输方式：冻存运输
品系：BALB/c
组织来源：ascites
细胞类型：其他细胞类型
是否是肿瘤细胞：0
物种来源：小鼠
年限：adult
数量：大量
细胞形态：单核细胞/巨噬细胞
规格：0.5ml
Designations 统一别名 : RAW 264.7
Depositors:WC Raschke
Biosafety Level 生物安全等级 :2
Shipped 提供形式 :frozen
Medium & Serum: See Propagation
Growth Properties:adherent
Organism:Mus musculus
Morphology:monocyte/macrophage
Source:
Strain: BALB/c
Tissue: ascites
Disease: Abelson murine leukemia virus-induced tumor
Cell Type:macrophage; Abelson murine leukemia virus transformed
Cellular Products:lysozyme
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material.
Anyone purchasing ATCC material is ultimately responsible for obtaining the permits.
Please click here for information regarding the specific requirements for shipment to your location.
Applications 用途 :Biological response transfection host
Receptors:complement (C3) [1207 ] Antigen Expression:H-2d
Age:adult
Gender:male
Comments 注释 :This line was established from a tumor induced by Abelson murine leukemia virus. They are negative for surface immunoglobulin (sIg-), Ia (Ia-) and Thy-1.2 (Thy-1.2) This line does not secrete detectable virus particles and is negative in the XC plaque formation assay.
The cells will pinocytose neutral red and will phagocytose latex beads and zymosan. They are capable of antibody dependent lysis of sheep erythrocytes and tumor cell targets.
LPS or PPD treatment for 2 days stimulates lysis of erythrocytes but not tumor cell targets. Data communicated in Feb. 2007 by Dr Janet W. Hartley, indicates the expression of infectious ecotropic MuLV closely related, if not identical,to the Moloney MuLV helper virus used in the original virus inoculum. The cells also express polytropic MuLV,unsurprisingly based on the mouse passage history of the virus stocks [ PubMed 18177500].
Propagation:ATCC complete growth medium:The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002.
To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere 需氧情况 : air, 95%; carbon dioxide (CO2),5%
Temperature 培养温度 : 37.0°C
Subculturing:
Protocol: Subcultures are prepared by scraping.
For a 75 cm2 flask, remove all but 10 ml culture medium (adjust amount accordingly for other culture vessels).
Dislodge cells from the flask substrate with a cell scraper; aspirate and add appropriate aliquots of the cell suspension into new culture vessels.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: Replace or add medium every 2 to 3 days.
Preservation:
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature 保藏温度 : liquid nitrogen vapor phase
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002 recommended serum:ATCC 30-2020
注意事项：
1. 收到细胞后首先观察细胞瓶是否完好，培养液是否有漏液、浑浊等现象，若有上述现象发生请及时和我们联系。
2. 仔细阅读细胞说明书，了解细胞相关信息，如细胞形态、所用培养基、血清比例、所需细胞因子等，确保细胞培养条件一致。若由于培养条件不一致而导致细胞出现问题，责任由客户自行承担。
3. 用 75%酒精擦拭细胞瓶表面，显微镜下观察细胞状态。因运输问题贴壁细胞会有少量从瓶壁脱落，将细胞置于培养箱内静置培养 4~6 小时,再取出观察。此时多数细胞均会贴壁，若细胞仍不能贴壁请用台盼蓝染色测定细胞活力，如果证实细胞活力正常，请将细胞离心后用新鲜培养基再次贴壁培养；如果染色结果显示细胞无活力，请拍下照片及时和我们联系，信息确认后我们为您再免费寄送一次。
4. 静置细胞贴壁后，请将细胞瓶内的培养基倒出，留 6~8mL 维持细胞正常培养，待细胞汇合度 80%左右时正常传代。
5. 请客户用相同条件的培养基用于细胞培养。培养瓶内多余的培养基可收集备用，细胞传代时可以一定比例和客户自备的培养基混合，使细胞逐渐适应培养条件。
6. 建议客户收到细胞后前 3 天各拍几张细胞照片，记录细胞状态，便于和中国微生物菌种查询网（www.biobw.org）技术部沟通交流。由于运输的原因，个别敏感细胞会出现不稳定的情况，请及时和我们联系，告知细胞的具体情况，以便我们的技术人员跟踪回访直至问题解决。