编号：ATCC 9763
生物体：酿酒酵母NRRL Y-567
产品形式：悬浮在适当的冷冻保护剂中的酵母细胞有效期
储存条件：2°C至8°C冷冻干燥培养，低湿度; - 冷冻培养70°C或更冷
注意：不要将冷冻小瓶存放在带有除霜循环的冷冻箱中，因为这会使小瓶暴露在升高的温度下。 气相液氮（约 - 196°C）是长期储存（超过一个月）的首选。
| 测试 |
规范 |
结果 |
|
可行性 |
将样品材料接种在非选择性培养基上，孵育并检查滴度。 结果≥103cfu /小瓶。 |
6.6 x 106 cfu /小瓶 |
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细胞和集落形态整合 |
将样品材料接种并在非选择性培养基上生长。 为了形态符合物种描述（即参考文献1），目测检查平板/斜面上的菌落，并在200-400倍放大倍数下在显微镜下检查细胞。 文化与适当的物种描述一致。 |
通过 |
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纯度 |
将样品材料接种在细菌生长培养基（液体）中，并在25℃和37℃下孵育至少72小时。 沉淀物（如果有的话）在显微镜下以1000倍放大率检查。 没有明显的细菌细胞或其他异常细胞。 |
通过 |
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表型测试 |
样品由bioMérieuxVITEK?评估.2。结果与被测生物一致。 |
通过 |
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基因型检测 |
通过D1 / D2基因区域测序评估样品。 结果与被测试的生物体是可接受的匹配。 |
通过 |
Saccharomyces cerevisiae Meyen ex E.C. Hansen 拉丁名
(ATCC? 9763?) 统一编号
Deposited As Saccharomyces cerevisiae Hansen Classification Saccharomycetes, Saccharomycetidae, Saccharomycetales, Saccharomycetaceae, Saccharomycetaceae, Saccharomyces, cerevisiae
Strain Designations 菌株别名 NRRL Y-567 [CBS 2978, CBS 5900, CCY 21-4-48, CCY 21-4-54, NCTC 10716, NCTC 7239, NCYC 87, Pattee 6, PCI M-50]
Alternate State Candida robusta Diddens et Lodder
Application 用途 Assay of amphotericin； Assay of amphotericin B fungizone ；Assay of anisomycin； Assay of antifongine ；Assay of candicidin ；Assay of natamycin pimaricin, tennecetin；Assay of nystatin fungicidin； Media testing ；Produces arginase； Produces ethyl alcohol ethanol Food testing ；Pharmaceutical and Personal Care； Produces histone deacetylase ；Produces nicotinic acid niacin ；Sterility testing； Susceptibility testing ；Testing disinfectants Testing fungicides； Produces ethanol from saccharified whole corn mash；Reference strain for performance testing culture media listed by the ISO TC 34 SC 9 Joint Working Group 5 in the ISO 11133 and by the Working Party on Culture Media of the International Committee on Food Microbiology and Hygiene (ICFMH-WPCM)
Biosafety Level 生物安全等级 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format 提供形式 freeze-dried
Storage Conditions 保藏条件
Frozen 冷冻物: -80℃ or colder
Freeze-Dried 冻干物: 2℃ to 8℃
Live Culture 活物: See Propagation Section
Type Strain 模式菌株 no
Preceptrol? yes Ploidy polyploid Ref
Comments 注释 Invertase activity Oligosaccharide transfer in microsomes Deoxy-trehalose synthesis Morphology On YM agar after 3 days at 25℃, colonies are cream colored, smooth, usually flat, occasionally raised in the centerpart. Older colonies may be slightly tan and opaque. Cells are ovoid, globose, budding, usually isolated or clustered, 3.0-8.0 by 5.0-10.0 ?m.
Medium 培养基 ATCC? Medium 200: YM agar or YM broth
ATCC? Medium 28: Emmons' modification of Sabouraud's agar
ATCC? Medium 1245: YEPD
Growth Conditions 保藏条件
Temperature 培养温度: 30℃
Atmosphere 需氧情况: Typical aerobicSequenced Data 18S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and 26S ribosomal RNA gene, partial sequence TATTCATTAAATTTTTGTCAAAAACAAGAATTTTCGTAACTGGAAATTTTAAAATATTAAAAACT
TTCAACAACGGATCTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTAATG
GAATTGCAGAATTCCGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAG
GGGGCATGCCTGTTTGAGCGTCATTTCCTTCTCAAACATTCTGTTTGGTAGTGAGTGATACTCT
TTGGAGTTAACTTGAAATTGCTGGCCTTTTCATTGGATGTTTTTTTTTTCCAAAGAGAGGTTTCT
CTGCGTGCTTGAGGTATAATGCAAGTACGGTCGTTTTAGGTTTTACCAACTGCGGCTAATCTTTT
TTATACTGAGCGTATTGGAACGTTATCGATAAGAAGAGAGCGTCTAGGCGAACAA D1D2 region of the 26S ribosomal RNA gene ATATCAATAAGCGGAGGAAAAGAAACCAACCGGGATTGCCTTAGTAACGGCGAGTGAAGCGG
CAAAA
GCTCAAATTTGAAATCTGGTACCTTCGGTGCCCGAGTTGTAATTTGGAGAGGGCAACTTTGGG
GCCGTTCCTTGTCTATGTTCCTTGGAACAGGACGTCATAGAGGGTGAGAATCCCGTGTGGC
GAGGAGTGCGGTTCTTTGTAAAGTGCCTTCGAAGAGTCGAGTTGTTTGGGAATGCAGCTCTAA
TGGGTGGTAAATTCCATCTAAAGCTAAATATTGGCGAGAGACCGATAGCGAACAAGTACAGT
GATGGAAAGATGAAAAGAACTTTGAAAAGAGAGTGAAAAAGTACGTGAAATTGTTGAAAGG
GAAGGGCATTTGATCAGACATGGTGTTTTGTGCCCTCTGCTCCTTGTGGGTAGGGGAATCTCG
CATTTCACTGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCATAGGAATGTAGCTTGCCTC
GGTAA
GTATTATAGCCTGTGGGAATACTGCCAGCTGGGACTGAGGACTGCGACGTAAGTCAAGGATG
CTGGCATAATGGTTATATGCCGC
Morphology On YM agar after 3 days at 25℃, colonies are cream colored, smooth, usually flat, occasionally raised in the centerpart. Older colonies may be slightly tan and opaque. Cells are ovoid, globose, budding, usually isolated or clustered, 3.0-8.0 by 5.0-10.0 ?m.