编号：ATCC 9179
批号：63423352
生物：青霉（Penicillium rubens）NRRL 832
产品形式：孢子和菌丝体悬浮在适当的冷冻保护剂中有效期：不适用
储存条件：2°C至8°C冷冻干燥培养，低湿度; - 冷冻培养70°C或更冷
注意：不要将冷冻小瓶存放在带有除霜循环的冷冻箱中，因为这会使小瓶暴露在升高的温度下。 气相液氮（约 - 196°C）是长期储存（超过一个月）的首选。
测试/方法规格结果
可行性：将样品材料接种在非选择性培养基上，培养并检查活细胞。文化显示增长并且是可行的。通过
细胞和集落形态整合：将样品材料接种并在非选择性培养基上生长。为了形态符合物种描述（即参考文献1），目测检查平板/斜面上的菌落，并在200-400倍放大倍数下在显微镜下检查细胞。文化与适当的物种描述一致。通过
纯度：将样品材料接种在细菌生长培养基（液体）中，并在25℃和37℃下培养最少48小时。
沉淀物：在显微镜下以1000倍放大率检查。没有明显的细菌细胞或其他异常细胞。通过
基因型检测通过ITS和部分28S测序评估样品。结果与被测试的生物体是可接受的匹配。通过
ATCC?培养基28：Emmons对Sabouraud琼脂ATCC?培养基312的修饰：Czapek的琼脂
ATCC?培养基336：马铃薯葡萄糖琼脂（PDA）
生长条件温度：25°C至30°C气氛：典型的有氧运动
推荐程序
对于freezedry（冻干）安瓿：
1.从单个无菌蒸馏水试管（5至6 mL）中，用无菌移液管取出约0.5至1.0 mL，直接涂于颗粒上。搅拌形成悬浮液。
2.将悬浮液无菌转移回无菌蒸馏水的试管中。
3.让试管在室温（25℃）下静置至少2小时;更长时间（例如，过夜）再水化可以增加一些真菌的活力。
4.将悬浮液混合均匀。使用几滴（或根据需要进行稀释）接种推荐的固体或液体培养基。包括不接受接种物的对照。
5.在推荐的繁殖条件下孵育接种物。
6.定期检查接种物/菌株的生长情况。在孵育2至3天后，活力通常是显着的。然而，显着增长所需的时间因品种而异。
Penicillium rubens Biourge拉丁名 (ATCC? 9179?) 统一编号
Deposited As Penicillium notatum Westling
Strain Designations 菌株别名 NRRL 832 [ATCC 7813, B 69, CBS 197.46, IMI 17968, QM 940]
Application 用途 Produces penicillin； Produces phosphogluconate dehydrogenase 6-phosphogluconate dehydrogenase
Biosafety Level 生物安全等级 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format 提供形式 freeze-dried
Storage Conditions 保藏条件
Frozen 冷冻物: -80℃ or colder
Freeze-Dried 冻干物: 2℃ to 8℃
Live Culture 活物: See Propagation Section
Type Strain 模式菌株 no
Preceptrol? yes
Comments Prototype strain for production of penicillin in submerged culture The strain was reidentified as Penicillium rubens based on the multiloci DNA sequence analysis
Medium 培养基 ATCC? Medium 28: Emmons' modification of Sabouraud's agar
ATCC? Medium 312: Czapek's agar
ATCC? Medium 336: Potato dextrose agar (PDA)
Growth Conditions 生长条件
Temperature 培养温度: 25℃ to 30℃
Atmosphere 需氧情况: Typcial aerobic
Sequenced Data 18S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and 28S ribosomal RNA gene, partial sequence GGTTTCCGTAGGTGAACCTGCGGAAGGATCATTACCGAGTGAGGGCCCTCTGGGTCCAACCTCCCACCCGTGTTTATT
TTACCTTGTTGCTTCGGCGGGCCCGCCTTAACTGGCCGCCGGGGGGCTTACGCCCCCGGGCCCGCGCCCGCCGAAGA
CACCCTCGAACTCTGTCTGAAGATTGTAGTCTGAGTGAAAATATAAATTATTTAAAACTTTCAACAACGGATCTCTTGGTTCCG
GCATCGATGAAGAACGCAGCGAAATGCGATACGTAATGTGAATTGCAAATTCAGTGAATCATCGAGTCTTTGAACGCACATTG
CGCCCCCTGGTATTCCGGGGGGCATGCCTGTCCGAGCGTCATTTCTGCCCTCAAGCACGGCTTGTGTGTTGGGCCCCGTC
CTCCGATCCCGGGGGACGGGCCCGAAAGGCAGCGGCGGCACCGCGTCCGGTCCTCGAGCGTATGGGGCTTTGTCACCC
GCTCTGTAGGCCCGGCCGGCGCTTGCCGATCAACCCAAATTTTTATCCAGGTTGACCTCGGATCAGGTAGGGATACCCGCT
GAACTTAAGCATATCAATAA Beta-tubulin (TUB2) gene, partial sequence GATCCCCTCCAGACGCGTCTTTTGTTTTCATATGGCCCCTGAGCCACGACCCCACCCCCAACAGATCTTTTGCTAAC
TTGCTCTA
GGTTCACCTCCAAACCGGCCAGTGTGTAAGTTCAACATGGAACATTCTTGGAAACATTCTTCGATTCGTGGGACT
AAACTGGAATTGGGTTATAGGGTAACCAAATTGGTGCCGCTTTCTGGTAAGTCTCGGAGCTTTTTTTTTCGCGTTGGG
TATCAATTGACAAGTTGCTAACTGGATTACAGGCAAACCATCTCTGGCGAGCACGGTCTCGATGGCGATGGACAGTA
AGTTTAACAGTGATAGGGATTCTGGTGGATCACACGTCTGATATCTTGCTAGGTACAATGGTACCTCCGACCTCCAGC
TCGAGCGTATGAACGTCTACTTCAACCATGTGAGTACAATGACTGGGAATCGATTGATTGTGCATCATCTGATCGGAC
GTTTTTCTTTGACAATCTAGGCCAGCGGTGACAAGTACGTTCCCCGTGCCGTTCTGGTCGATTTGGAGCCCGGTACC
ATGGATGCTGTCCGCTCCGGTCCCTTCGGCAAGCTTTTCCGCCCCGACAACTTCGTCTTCGGTCAGTCCGGTGCTG
GTAACAACTGGGCCAAGGGTCACT D1D2 region of the 28S ribosomal RNA gene ATATCAATAAGCGGAGGAAAAGAAACCAACAGGGATTGCCCCAGTAACGGCGAGTGAAGCGGCAAGAGCTCAAATT
TGAAAGCTGGCTCCTTCGGGGTCCGCATTGTAATTTGTAGAGGATGCTTCGGGAGCGGTCCCCAT
CTAAGTGCCCTGGAACGGGACGTCATAGAGGGTGAGAATCCCGTATGGGATGGGGTGTCCGCGCCCGTGTGAAGC
TCCTTCGACGAGTCGAGTTGTTTGGGAATGCAGCTCTAAATGGGTGGTAAATTTCATCTAAAGCTAAATATTGGCCGG
AGACCGATAGCGCACAAGTAGAGTGATCGAAAGATGAAAAGCACTTTGAAAAGAGAGTTAAAAAGCACGTGAAATTG
TTGAAAGGGAAGCGCTTGCGACCAGACTCGCTCGCGGGGTTCAGCCGGCATTCGTGCCGGTGTACTTCCCCGCGG
GCGGGCCAGCGTCGGTTTGGGCGGTCGGTCAAAGGCCCTCGGAAGGTAACGCCCCTAGGGGCGTCTTATAGCCG
AGGGTGCAATGCGACCTGCCTAGACCGAGGAACGCGCTTCGGCTCGGACGCTGGCATAATGGTCGTAAACGAC
Name of Depositor 寄存人 NRRL
Chain of Custody 来源国家 ATCC <-- NRRL <-- C Thom B 69
Isolation 分离基物 Contaminant in culture labeled 'P. lacticus Maze & Perrier' from Ph. Biourge 69, Washington, DC
Cross References Nucleotide (GenBank) : KU729086 ITS including 5.8S rRNA gene
Nucleotide (GenBank) : KU729184 D1/D2 region of 28S rRNA gene
Nucleotide (GenBank) : KU896998 beta-tubulin (TUB2) gene
References参考文献 Houbraken J, et al. New penicillin-producing Penicillium species and an overview of section Chrysogena. Persoonia 29: 78-100, 2012. PubMed: 23606767
Miller HM, Shepherd MG. Purification and properties of 6-phosphogluconate dehydrogenase from Penicillium duponti and Penicillium notatum. Can. J. Microbiol. 18: 1289-1298, 1972. PubMed: 5052896
Moyer AJ, Coghill RD. Penicillin. IX. The laboratory scale production of penicillin in submerged cultures by Penicillium notatum Westling (NRRL 832). J. Bacteriol. 51: 79-93, 1946.
Chou H, et al. Transaldolases are novel and immunoglobulin E cross-reacting fungal allergens. Clin. Exp. Allergy 41: 739-749, 2011. PubMed: 21488999
Houbraken J, Frisvad JC, Samson RA. Fleming's penicillin producing strain is not Penicillium chrysogenum but P. rubens. IMA Fungus 2: 87-95, 2011. PubMed: 22679592
Chow LP, et al. Characterization of Pen n 13, a major allergen from the mold Penicillium notatum. Biochem. Biophys. Res. Commun. 269: 14-20, 2000. PubMed: 10694469