【成份】
1063 ANAEROBIC SEAWATER MEDIUM
Solution A:
KH2PO4 0.2 g
NH4Cl 0.25 g
NaCl 20.0 g
MgCl2 . 6 H2O 3.0 g
KCl 0.5 g
CaCl2 . 2 H2O 0.15 g
Na-resazurin solution (0.1% w
) 0.5 ml
蒸馏水 940.0 ml
Solution B:
Trace element solution SL-10 1.0 ml
Solution C:
Vitamin solution 1.0 ml
Solution D:
Selenite-tungstate solution 1.0 ml
Solution E:
NaHCO3 3.5 g
蒸馏水 50.0 ml
Solution F:
D-glucose 2.0 g
Yeast extract 0.5 g
蒸馏水 1.0 L
Solution G:
Na2S·9H2O 0.3 g
蒸馏水 10.0 ml
Sparge solution A with 80% N2 and 20% CO2 gas mixture for 30 – 45 min to make it anoxic
distribute under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Solutions B
D and G are autoclaved separately under 100% N2 gas. Solutions C and F are prepared under 100% N2 gas and sterilized by filtration. Solution E is autoclaved under 80% N2 and 20% CO2 gas atmosphere. To complete the medium appropriate amounts of solutions B to G are added to the sterile solution A in the sequence as indicated. Adjust pH of complete medium to 7.2 - 7.4
if necessary.
Selenite-tungstate solution:
NaOH 0.5 g
Na2SeO3·5 H2O 3.0 mg
Na2WO4·2 H2O 4.0 mg
蒸馏水 1.0 L
Trace element solution SL-10:
HCl (25%; 7.7 M) 10.0 ml
FeCl2·4 H2O 1.5 g
ZnCl2 70.0 mg
MnCl2·4 H2O 100.0 mg
H3BO3 6.0 mg
CoCl2·6 H2O 190.0 mg
CuCl2 ·2 H2O 2.0 mg
NiCl2·6H2O 24.0 mg
Na2MoO4·2H2O 36.0 mg
蒸馏水 990.0 ml
First dissolve FeCl2 in the HCl
then dilute in water
add and dissolve the other salts. Finally make up to 1000.0 ml.
Seven vitamins solution:
Vitamin B12 100.0 mg
p-Aminobenzoic acid 80.0 mg
D(+)-Biotin 20.0 mg
Nicotinic acid 200.0 mg
Calcium pantothenate 100.0 mg
Pyridoxine hydrochloride 300.0 mg
Thiamine-HCl x· 2 H2O 200.0 mg
蒸馏水 1.0 L