【成份】
1062
D-Glucose 2.0 g
Tryptone 2.0 g
Na-resazurin solution (0.1% w
) 0.5 ml
L-Cysteine-HCl·H2O 0.25 g
Trace element solution SL-10 10.0 ml
Trace element solution(见培养基0940) 25.0 ml
Vitamin solution(见培养基0901) 10.0 ml
蒸馏水 1000.0 ml
Dissolve ingredients (except cysteine
trace elements and vitamins)
sparge medium with 100% N2 gas for 30 – 45 min to make it anoxic. Then add cysteine
adjust pH to 7.0
dispense medium under same gas atmosphere in anoxic Hungate-type tubes or serum vials and autoclave. After sterilization add trace elements and vitamins from sterile anoxic stock solutions prepared under 100% N2 gas. Vitamins should be sterilized by filtration. Check pH of complete medium and adjust to 7.0
if necessary.
Trace element solution SL-10:
HCl (25%; 7.7 M) 10.0 ml
FeCl2·4 H2O 1.5 g
ZnCl2 70.0 mg
MnCl2·4 H2O 100.0 mg
H3BO3 6.0 mg
CoCl2·6 H2O 190.0 mg
CuCl2 ·2 H2O 2.0 mg
NiCl2·6H2O 24.0 mg
Na2MoO4·2H2O 36.0 mg
蒸馏水 990.0 ml
First dissolve FeCl2 in the HCl
then dilute in water
add and dissolve the other salts. Finally make up to 1000.0 ml.