pGL4.43载体基本信息
载体名称:pGL4.43 ; pGL4.43[luc2P/XRE/Hygro]
质粒类型:信号通路报告载体;哺乳动物载体;萤火虫荧光素酶报告载体
高拷贝/低拷贝:--
克隆方法:限制性内切酶,多克隆位点
启动子:Minimal Promotor
载体大小:6067 bp
5' 测序引物及序列:--
3' 测序引物及序列:--
载体标签:luc2P
载体抗性:氨苄青霉素
筛选标记:潮霉素(Hygromycin)
克隆菌株:TOP10等常规菌株
宿主细胞(系):HEK293等
备注:pGL4.43[luc2P/XRE/Hygro]载体是信号通路报告载体;含异己物应答元件XRE;含萤火虫荧光素酶报告基因luc2P。
产品目录号:E3751
稳定性:稳表达
组成型/诱导型:组成型
病毒/非病毒:非病毒
pGL4.43载体质粒图谱和多克隆位点信息
pGL4.43载体简介
pGL4.43载体简介
The pGL4.43[luc2P/XRE/Hygro] Vector contains three copies of a xenobiotic response element (XRE) that drives transcription of the luciferase reporter gene luc2P (Photinus pyralis). luc2P is a synthetically-derived luciferase sequence with humanized codon optimization that is designed for high expression and reduced anomalous transcription. The luc2P gene contains hPEST, a protein destabilization sequence, which allows luc2P protein levels to respond more quickly than those of luc2 to induction of transcription. The vector backbone contains an ampicillin resistance gene to allow selection in E. coli and a gene for hygromycin resistance to allow selection of stably transfected mammalian cell lines.