NCTC clone 3526[derivative of LLC-MK2] 拉丁名

(ATCC® CCL-7.2™) 统一编号 

Organism Macaca mulatta, monkey, rhesus

Product Format 提供形式 frozen

Morphology epithelial

Culture Properties adherent

Biosafety Level 生物安全等级 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Age adult

Applications The cells are positive for keratin by immunoperoxidase staining.

Genes Expressed The cells are positive for keratin by immunoperoxidase staining. keratin,The cells are positive for keratin by immunoperoxidase staining.

Cellular Products keratin Comments Grows in chemically defined medium. The cells are positive for keratin by immunoperoxidase staining.

Complete Growth Medium NCTC 135 medium, 100% Subculturing Subcultivation Ratio: A subcultivation ratio of 1:2 is recommended Medium Renewal: 3 times per week Subcultures are prepared by scraping.

Remove old medium, add fresh, dislodge cells from the floor of the flask, aspirate and dispense into new flasks.

Name of Depositor 寄存人 VJ Evans

References 参考文献 Evans VJ, et al. Chemically defined media for cultivation of long-term cell strains from four mammalian species. Exp. Cell Res. 36: 439-474, 1964. PubMed: 14242231

J. Natl. Cancer Inst. 29: 749, 1962.

Hull RN, Tritch OJ. Characterization of cell strains by viral susceptibility. Natl. Cancer Inst. Monogr. 7: 161-172, 1962. PubMed: 14449902 .

. Am. J. Hyg. 70: 275, 1959.