年限:70 years
数量:大量
ATCC Number:CRL-2260?
相关疾病:其他疾病
组织来源:ascites
运输方式:冻存运输
细胞形态:淋巴样
免疫类型:kappa
生长状态:悬浮生长
细胞类型:B淋巴细胞
是否是肿瘤细胞:1
物种来源:人
规格:60 次 Designations: HT
Depositors: ?M Beckwith
Isotype: kappa
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Homo sapiens
Morphology:lymphoblast
Source: Tissue: ascites
Disease: diffuse mixed lymphoma
Cell Type: B lymphoblast;
Cellular Products:immunoglobulin; kappa light chain
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Isolation: Isolation date: 1983
Antigen Expression:CD19 +; CD20 +; CD21 +; CD22 +; Hle-1 +; HLA DQ +; HLA DR +; CD25 -; T cell receptor (TCR) -
DNA Profile (STR):Amelogenin: X,Y
CSF1PO: 10
D13S317: 13,14
D16S539: 11,13
D5S818: 11,13
D7S820: 8,10
THO1: 6,7
TPOX: 11
vWA: 17,18
Age: 70 years
Gender: male
Ethnicity: Caucasian
Comments:HT is a human B cell lymphoma cell line originated in 1983 by Walter J. Urba and Dan L. Longo.
HT cells express mRNA for both IgM and kappa and express low levels of kappa on the cell surface.
Exposure of HT cells to protein kinase C activating phorbol esters such as PMA and PdBu induced profound growth inhibition.
HT cells have been reported to be Epstein-Barr virus genome negative.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Protocol: Cultures can be maintained by addition or replacement of medium. Establish new cultures at 2 X 10(5) viable cells/ml and maintain between 1 X 10(5) and 1 X 10(6).
Medium Renewal: Every 2 to 3 days
Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
References: 22555: Beckwith M, et al. Phorbol ester-induced, cell-cycle-specific, growth inhibition of human B-lymphoma cell lines. J. Natl. Cancer Inst. 82: 501-509, 1990. PubMed: 2313723