运输方式:冻存运输
ATCC Number:CRL-11102?
相关疾病:其他疾病
细胞类型:B淋巴细胞
是否是肿瘤细胞:0
物种来源:人
生长状态:悬浮生长
数量:大量
细胞形态:淋巴样
免疫类型:IgG; kappa light chain
规格:0.3ml Designations: DS-1
Depositors: ?The United States of America
Isotype: IgG; kappa light chain
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Homo sapiens
Morphology:lymphoblast
Source: Disease: lymphangiectasia
Derived from metastatic site: pleural effusion
Cell Type: B lymphocyte;
Cellular Products:surface immunoglobulin
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Receptors:interleukin 6 (IL-6)
Antigen Expression:HLA Class I, (100%); HLA Class II (38%); plasma cell antigen (PCA) (99%); CD2 (<5%); CD3 (<5%); CD4 -; CD5 -; CD7 -; CD8 -; CD20 -; CD30 -; CD38 -; CD45 -
DNA Profile (STR):Amelogenin: X
CSF1PO: 12
D13S317: 9,12
D16S539: 9,10
D5S818: 11,14
D7S820: 8,14
THO1: 6,9.3
TPOX: 9,11
vWA: 18,19
Comments:The DS-1 cell line was established from cells in the pleural effusion from an immunodeficient patient with intestinal lymphangiectasia.
DS-1 is IL-6 dependent.
Propagation: ATCC complete growth medium: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate supplemented with 10 U/ml IL-6, 90%; fetal bovine serum, 10%
Temperature: 37.0°C
Subculturing: 55-X ).
Medium Renewal: Every 2 to 3 daysATCC Protocol: Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 X 10 (5) cells/ml. Maintain cultures between 1 X 10 (5) and 2 X 10 (6) cells/ml.Recovery of this line from the frozen state is improved by using a feeder layer of irradiated MRC-5 cells (
Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Bioreactive Factors: Growth Factors: interleukin 6 (IL-6)
References: 22116: Bock GH, et al. Monoclonal antibody recognizing a surface molecule on a subset of antigen-stimulated T cells and on certain malignancies of T and B cell origin. US Patent 5,556,947 dated Sep 17 1996