运输方式:冻存运输
数量:大量
细胞形态:成纤维样
器官来源:其他
ATCC Number:CCL-169?
相关疾病:正常
细胞类型:成纤维细胞
生长状态:贴壁生长
是否是肿瘤细胞:0
物种来源:其他
年限:15 to 17 days gestation
规格:0.1ml Designations: CGBQ
Depositors: ?HG Coon
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Anser anser
Morphology:fibroblast
Source: Organ: sternum
Disease: normal
Cell Type: fibroblast
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Virus Susceptibility:Herpes simplex virus
Sindbis virus
Vesicular stomatitis virus
Human poliovirus 1
Cytogenetic Analysis:range 80 to 145
Exact chromosome count to determine the modal number is difficult to achieve owing to the large number of microchromosomes which complicate the picture. There are 20-27 macrochromosomes.
Age: 15 to 17 days gestation
Propagation: ATCC complete growth medium: Ham's F12K medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 85%; fetal bovine serum, 15%
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Protocol:
- Remove and discard culture medium.
- Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
- Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal.
- Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
- Add appropriate aliquots of the cell suspension to new culture vessels.
- Incubate cultures at 37?C.
Subcultivation Ratio: A subcultivation ratio of 1:3 is recommended
Medium Renewal: 2 to 3 times per week
Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) glycerol
Storage temperature: liquid nitrogen vapor phase
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2004
recommended serum:ATCC 30-2020
References: 21858: . . Carnegie Inst. Wash. Year Book 67: 421, 1968.
22582: Coon HG, Weiss MC. A quantitative comparison of formation of spontaneous and virus- produced viable hybrids. Proc. Natl. Acad. Sci. USA 62: 852-859, 1969. PubMed: 4308097