ATCC Number:CRL-1729?
运输方式:冻存运输
生长状态:悬浮生长
是否是肿瘤细胞:0
物种来源:小鼠
数量:大量
细胞形态:淋巴样
免疫类型:IgG1
规格:0.2ml Designations: MYC 1-9E10.2 [9E10]
Depositors: ?J Bishop, GI Evan
Isotype: IgG1
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology:lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products:immunoglobulin; monoclonal antibody; against human myc (c-myc) protein
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Applications:Mice were immunized with a synthetic peptide derived using the sequence of the human c-myc gene product.
Tested and found negative for ectromelia virus (mousepox).
Comments:Mice were immunized with a synthetic peptide derived using the sequence of the human c-myc gene product.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody precipitates the p62 c-myc protein, but does not react with mouse or chicken c-myc.
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
References: 1189: Evan GI, et al. Isolation of monoclonal antibodies specific for human c-myc proto-oncogene product. Mol. Cell. Biol. 5: 3610-3616, 1985. PubMed: 3915782
29119: Van Ewijk WV, et al. Subtractive isolation of phage-displayed single-chain antibodies to thymic stromal cells by using intact thymic fragments. Proc. Natl. Acad. Sci. USA 94: 3903-3908, 1997. PubMed: 9108077
33026: Cheng M, et al. ERK3 is a constitutively nuclear protein kinase. J. Biol. Chem. 271: 8951-8958, 1996. PubMed: 8621539