细胞形态:淋巴样
生长状态:悬浮生长
数量:大量
ATCC Number:TIB-49?
相关疾病:其他疾病
是否是肿瘤细胞:0
物种来源:小鼠
品系:C57BL/6J
运输方式:冻存运输
规格:0.2ml Designations: C1498
Depositors: ?P Ralph
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Mus musculus
Morphology:lymphoblast
Source: Disease: acute myeloid leukemia
Strain: C57BL/6J
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Antigen Expression:The cells do not express B7-1.
Gender: female
Comments:They are resistant to 0.1 mM cortisol.
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Protocol: Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can beestablished by centrifugation with subsequent resuspension in fresh medium at 1 to 3 x 10 exp5 viable cells/ml. A saturation density of 1 to 2 X 10 exp6 is obtainable.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Preservation: Freeze medium: Complete growth medium 92.5%; DMSO, 7.5%
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
References: 1104: Ralph P. Retention of lymphocyte characteristics by myelomas and theta+-lymphomas: sensitivity to cortisol and phytohemagglutinin. J. Immunol. 110: 1470-1475, 1973. PubMed: 4541304
22528: Ralph P, Nakoinz I. Inhibitory effects of lectins and lymphocyte mitogens on murine lymphomas and myelomas. J. Natl. Cancer Inst. 51: 883-890, 1973. PubMed: 4542714
30399: Boyer MW, et al. Thr role of B7 costimulation by murine acute myeloid leukemia in the generation and function of a CD8+ T-cell line with potent in vivo graft-versus-leukemia properties. Blood 89: 3477-3485, 1997. PubMed: 9129056