生长状态：悬浮生长
运输方式：冻存运输
ATCC Number：CRL-1992?
细胞形态：淋巴样
数量：大量
是否是肿瘤细胞：0
物种来源：人
规格：0.5mg Designations: T2 (174 x CEM.T2)
Depositors: ?P Cresswell
Biosafety Level:2
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Homo sapiens
Morphology:lymphoblast
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Applications:transfection host (Roche Transfection Reagents )
Antigen Expression:HLA A2; CD7 +
DNA Profile (STR):Amelogenin: X
CSF1PO: 11, 12
D13S317: 11, 12
D16S539: 10, 12
D5S818: 12, 14
D7S820: 10, 11, 15
THO1: 6, 7, 9.3
TPOX: 8, 12
vWA: 14, 17, 20
Comments:This line is a variant of the T1 cell line (ATCC CRL-1991 ) produced by selection the SFR1-MI.3 monoclonal antibody (against a monomorphic determinant on HLA DR).
The cells do not express HLA DR and are Class II major histocompatibility (MHC) antigen negative.
The cells synthesize, but do not express, HLA B5.
T2 appears to have lost both copies of the CEMR.3 derived chromosome 6.
Together the T1 and T2 lines are useful for studying antigen processing and T cell recognition of class I MHC antigens.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
Temperature: 37.0°C
Subculturing: Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 3 X 10(5) viable cells/ml and maintain between 3 X 10(5) and 1 X 10(6) viable cells/ml.
Medium Renewal: Every 2 to 3 days
Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2005
recommended serum:ATCC 30-2020
References: 22647: Salter RD, et al. Genes regulating HLA class I antigen expression in T-B lymphoblast hybrids. Immunogenetics 21: 235-246, 1985. PubMed: 3872841
