Fowl adenovirus 3
VR-1854™
Product category
Viruses
Product type
Animal virus
Classification
Adenoviridae, Aviadenovirus, Fowl adenovirus D
Strain designation
IBH-2A (Tipton)
Isolation source
Liver of chicken with aplastic anemia and inclusion body hepatitis.
Applications
Infectious disease research
Respiratory disease research
Product format
Frozen
Storage conditions
-70°C or colder
General
Specific applications
Respiratory research
Characteristics
Comments
Derived by adaptation of primary cell culture product ATCC® VR-828™ to LMH cells (ATCC® CRL-2117™). ATCC® VR-828™ and ATCC® VR-1854™ have not been compared for sequence or infectivity in primary cell culture and tissue culture.
Handling information
Host
LMH (ATCC CRL-2117)
Effects
cell degeneration; cell rounding; CPE
Complete medium
Waymouth’s Medium (Gibco® 11220035) + 2% FBS (ATCC® 30-2020™)
Temperature
37°C
Atmosphere
95% Air, 5% CO2
Incubation
5-8 days at 37°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80% of the monolayer.
Recommendation for infection
Plate cells 18-24 hours prior to infection and infect when cultures are 70-80% confluent. Remove medium and inoculate with a small volume of virus (e.g., 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g., 0.1). Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere. End adsorption by adding virus growth medium.
Handling notes
Derived by adaptation of primary cell culture product ATCC® VR-828™ to LMH cells (ATCC® CRL-2117™). ATCC® VR-828™ and ATCC® VR-1854™ have not been compared for sequence or infectivity in primary cell culture and tissue culture.
Key abbreviations
°C, Degrees Celsius
CO2, Carbon dioxide
FBS, Fetal bovine serum
MOI, Multiplicity of infection
Quality control specifications
History
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