Human coronavirus 229E 病毒
(ATCC? VR-740?) 病毒编号
Classification Coronaviridae, Coronavirus
Agent Human coronavirus 229E
Strain 229E
Common Name Human coronavirus
Applications 用途 No hemolysis production. Respiratory research
Biosafety Level 生物安全等级 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format 提供形式 frozen 1 mL per vial
Storage Conditions 保藏方法 -70℃ or colder
Comments 注释 zPossibility of cross-reactions with other coronaviruses not thoroughly characterized at time of deposit (1973); does not cross-react with human myxoviruses.
Ether sensitive. No hemolysis production. Unstable at 24 & 37C; relatively stable at 10C. Harvest 26-50 hrs post adsorption before CPE is visible. Does not agglutinate chicken, guinea pig, human O, sheep, mouse or rat RBC. Size 89Mu (gradocol filtration). [RU1 are human embryonic lung fibroblasts developed by CDC]
Effect on Host
CPE, rounding and sloughing. Starts as individual cell rounding that spreads, but over time uninfected cells repopulate monolayer.
Recommended Host MRC-5 (ATCC? CCL-171?)
Growth Conditions 生长条件
Temperature 培养温度: 35℃
Recommendations for Infection: For best results cells should be 24 to 48 hours old and 80-90% confluent (not 100% confluent). Incubation: 2-7 days, a 5% CO2 in air atmosphere is recommended.
Effect on Host
CPE, rounding and sloughing. Starts as individual cell rounding that spreads, but over time uninfected cells repopulate monolayer. Name of Depositor HS Kaye Special Collection ATCC Source Nasal and throat swabs from man with mild upper respiratory illness
References 参考文献 Hamre D, Procknow JJ. A new virus isolated from the human respiratory tract. Proc. Soc. Exp. Biol. Med. 121: 190-193, 1966. PubMed: 4285768
