ATCC® Number:VR-1549™
Classification: Poxviridae, Orthopoxvirus
Agent: Vaccinia virus deposited as Vaccinia virus, Orthopoxvirus
Strain: Elstree (Lister Vaccine)
Original Source: derived from existing strain (Bacterially contaminated VR-862 (sheep isolate) was freeze-thawed three times, centrifuged to remove cellular debris, before filtering two times through a 0.45 micrometer filter and then one time through a 0.2 micrometer filter to inoculate a culture and create VR-1549.)
Depositors: JH Nakano, ATCC
Biosafety Level:2 [Vaccination recommended. (Vaccination recommended for laboratory staff manipulating vaccinia, monkeypox, or cowpox viruses [CDC/NIH Biosafety in Microbiological and Biomedical Laboratories, 4th ed, 1999 - stock no. 017-040-00547-4].)]
Shipped: frozen
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Incubation : Temperature: 37.0°C Duration: 3 days on chorioallantoic membrane; 3-4 days in Vero cells (ATCCCCL-81)
Effect : Yes, in vitro effects: Cytopathic effects Yes, in vivo effects: forms pocks on chorioallantoic membrane
Store at : -70 or colder
Related Products: formerly distributed as:ATCC VR-862