Human herpesvirus 2 拉丁名

(ATCC® VR-734™) 统一编号

Classification Herpesviridae, Simplexvirus 

Deposited As Herpes simplex type 2 

Agent Human herpesvirus 2 

Strain G 

Common Name Herpes simplex 2 

Applications Quality control 

Biosafety Level 生物安全等级 2 

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. 

Product Format 提供形式 frozen 1 mL per vial 

Storage Conditions 保存方法 -70°C or colder 

Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. 

Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.

Comments 注释 Deposited as Herpes simplex type 2 Vials contain total fluid and material from cell culture infected by virus. The vials will contain cellular debris and possibly cells containing cell-associated virus. 

Effect on Host CPE, refractile rounding, clumping, detachment 

Recommended Host Vero cells (ATCC CCL-81) 

Growth Conditions 生长条件 

Temperature 培养温度 : 34°C 

Recommendations for Infection: 

Plate cells 24 to 48 hours prior to infection and infect when cultures are 70-90% confluent. 

Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1). 

Adsorb 1-2 hours at 34°C in a humidified 5% CO2 atmosphere while rocking. End adsorption by adding virus growth medium. 

Incubation: 1 to 5 days in a humidified CO2 atmosphere 

Effect on Host CPE, refractile rounding, clumping, detachment

Name of Depositor 寄存人 B Roizman, Institut Merieux 

U.S. Patent 4,760,079 

U.S. Patent Number 4,760,079 

Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. 

Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. 

Special Collection NCRR Contract Source Human genital infection 

References 参考文献 Ejercito PM, et al. Characterization of herpes simplex virus strains differing in their effects on social behaviour of infected cells. J. Gen. Virol. 2: 357-364, 1968. PubMed: 4300104 

Roizman B, et al. Herpes simplex viruses I and II: a biochemical definition. Perspect. Virol. 8: 129-169, 1973.