Human herpesvirus 1 拉丁名
(ATCC® VR-1493™) 统一编号
Classification Herpesviridae, Simplexvirus
Deposited As Herpes simplex type 1
Agent Human herpesvirus 1
Strain KOS
Common Name Herpes simplex virus 1
Biosafety Level 生物安全等级 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format 提供形式 frozen 1 mL per vial
Storage Conditions 保存方法 -70°C or colder
Comments 注释 VR-1493™ was derived from VR-1487™ following treatment with a mycoplasma removal agent to remove mycoplasma contamination.
Antigenically cross-reactive with Human herpesvirus 2.
Effect on Host
CPE, syncytial formation, cell rounding, clumping, and sloughing
Recommended Host
Vero (ATCC® CCL-81™)
Growth Conditions 生长条件
Temperature 培养温度 : 37°C
Recommendations for Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 80-90% confluent.
Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.01).
Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum.
End adsorption by adding virus growth medium.
Incubation: 1-3 days
Effect on Host
CPE, syncytial formation, cell rounding, clumping, and sloughing
Name of Depositor 寄存人 PA Schaffer, University of Pennsylvania
Special Collection NCRR Contract
Source Lip lesion of human with cold sore
References 参考文献 Smith KO. Relationship between the envelope and the infectivity of herpes simplex virus. Proc. Soc. Exp. Biol. Med. 115: 814-816, 1964. PubMed: 14155835