ATCC® Number:VR-764™
Classification: Nidovirales, Coronaviridae, Coronavirus,Group 2
Agent: Murine hepatitis virus
Strain: MHV-A59
Original Source: Pool of liver and spleen suspension of BALB/c mice exhibiting rough coats and hunched position
Depositors: MJ Collins
Biosafety Level:2
Shipped: frozen
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Host Organism : NCTC clone 1469 cells (ATCCCCL-9.1)
Incubation : Atmosphere: 5% CO2 in air recommended Temperature: 37.0°C Duration: 18-24 hours; For best results cells should be 24 to 48 hours old and 80-90% confluent (not 100% confluent).
Effect : Yes, in vitro effects: Cytopathic effects in tissue culture
Store at : -70°C (or colder)
Comments : The MHV-A59 virus was isolated from BALB/c mice in which Moloney murine leukemia had been serially passaged by i.p. injection of homogenates of liver, spleen, thymus and lymph node. It is possible that the preparation still contains murine leukemia virus.