ATCC® Number:30295™
Organism: Polytomella caeca Pringsheim
Depositors: DL Wise
History: ATCC<<--DL Wise<<--UTEX 963 <<--- E.G. Pringsheim -- (?)
Biosafety Level:1
Shipped: freeze-dried
Growth Conditions: ATCCmedium 351: Hutner's medium for EuglenaTemperature: 25.0°C Duration: axenic Protocol: ATCCNO: 12910 SPEC: Aseptically add 0.5 ml of the growth medium to the inner shell vial containing the filter paper and incubate at 20C in the dark. Maintain the level of the medium in the vials at 0.5 ml during the incubation period. Observe daily for motile cells (motile cells should be observed within 6-8 days after rehydration). When numerous cells are evident, transfer the entire liquid contents of the vial (not the filter paper) to a tube of fresh medium and incubate at 25C.
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments: Preservation [4625] Life cycle [4779]
Classification: KINGDOM: Plantae
References: 4625: Reed RB, et al. Preservation of encysted Polytomella. J. Gen. Microbiol. 97: 29-34, 1976. PubMed: 186558 4779: Lewis E, et al. Life cycle of Polytomella caeca (phytomonadida, Polyblepharidae). J. Protozool. 21: 647-649, 1974.