ATCC® Number:PRA-107™
Organism: Acanthamoeba castellanii (Douglas) Page
Designations: 4CL
Isolation: clinical specimen - human (isolated from the contact lens case of an asymptomatic patient)Vienna Austria Isolation date: 1996
Depositors: J Walochnik
History: J Walochnik
Biosafety Level:2
Shipped: frozen
Growth Conditions: ATCCmedium 712: PYGTemperature: 25.0°C Duration: axenic Protocol: This strain is distributed as a freeze-dried preparation. See the general procedures for opening a freeze-dried vial. Aseptically add 0.5 ml of ice cold medium containing 12% (w/v) sucrose to the freeze-dried inner shell vial. Once the culture is completely rehydrated, aseptically add 1 ml of ATCCmedium 712 and distribute to a 16 X 125 mm plastic screw-capped test tube or a T-25 tissue culture flask containing 5.0 ml of the same medium. Incubate the test tube culture horizontally with the cap on tight. Trophozoites should be evident in 1-5 days. Interval: every month
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Classification: KINGDOM: Protozoa
References: 90184: Walochnik J, et al. Correlations between morphological, molecular biological, and physiological characteristics in clinical and nonclinical isolates of Acanthamoeba spp. Appl. Environ. Microbiol. 66: 4408-4413, 2000. PubMed: 11010891 90185: Walochnik J, et al. Immunological inter-strain crossreactivity correlated to 18S rDNA sequence types in Acanthamoeba spp. Int. J. Parasitol. 31: 163-167, 2001. PubMed: 11239936 90208: Hiti K, et al. Viability of Acanthamoeba after exposure to a multipurpose disinfecting contact lens solution and two hydrogen peroxide systems. Br. J. Ophthalmol. 86: 144-146, 2002. PubMed: 11815336