ATCC® Number:PVAS-766™
Organism: potato virus Y
Designation: potyvirus monoclonal antiserum [PTY10 cross-reactive monoclonal antiserum]
Depositors: R Jordan
Biosafety Level:1
Shipped: frozen
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments: Test animal: mouse Test method: ACP-ELISA (Reacts in ACP-ELISA, dot-blot and Western-blot assays). Hybridoma: 27G8H11 PTY 10 recognizes a potyvirus group-common, non-virion surface-located, conformation-independent, trypsin-resistant-core protein epitope found on at least 18 (of 30 tested) potyviruses. Reacts best with coat protein sub-unit rather than intact virus. Viruses tested, but reactivity not necessarily limited to: potato virus Y, pepper mottle, tobacco etch, tobacco vein mottling, turnip mosaic (7 of 8 strains), tulip breaking, sugarcane mosaic. Viruses tested, continued: asparagus virus 1, cowpea aphid-borne mosaic, sweet potato latent, watermelon mosaic virus 2, papaya ringspot virus-W, zucchini mosaic, plum pox.
References: 11878: Hammond J, Hammond RW. Molecular cloning, sequencing, and expression in Escherichia coli of the bean yellow mosaic virus coat protein gene. J. Gen. Virol. 70: 1961-1974, 1989. PubMed: 2671258 25431: McLaughlin MR, et al. Enzyme-linked immunosorbent assay of viruses infecting forage legumes. Phytopathology 74: 965-969, 1984. 25464: Vance VB, et al. The complete nucleotide sequence of pepper mottle virus genomic RNA: comparison of the encoded polyprotein with those of other sequenced potyviruses. Virology 191: 19-30, 1992. PubMed: 1413501 25561: Hammond J, Lawson RH. An improved purification procedure for preparing potyviruses and cytoplasmic inclusions from the same tissue. J. Virol. Methods 20: 203-217, 1988. PubMed: 3410929 25655: Uyeda I, et al. Relatedness of the nucleotide sequence of the 3'-terminal region of clover yellow vein potyvirus RNA to bean yellow mosaic potyvirus RNA. Intervirology 32: 234-245, 1991. PubMed: 1864719 31702: . Comparative potyvirus host range, serology, and coat protein peptide profiles of white lupin mosaic virus. Phytopathology 82: 566-571, 1992. 31732: Hammond J, et al. Use of polyclonal antisera and monoclonal antibodies to examine serological relationships among three filamentous viruses of sweet potato. Phytopathology 82: 713-717, 1992.