Influenza A virus (H3N2)
VR-1811 ™
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Product category
Viruses
Product type
Animal virus
Classification
Orthomyxoviridae, Alphainfluenzavirus
Strain designation
A/Virginia/ATCC6/2012
Genome sequenced strain
Yes
Isolation source
Clinical specimen from a patient.
Geographical isolation
United States; Virginia
Applications
Infectious disease research
Respiratory disease research
Product format
Frozen
Storage conditions
-70°C or colder
Characteristics
Comments
This item was isolated in cell culture by three passages in MDCK cells.
Handling information
Host
MDCK (NBL-2) (ATCC CCL-34)
Effects
cell rounding; cell sloughing; CPE
Complete medium
EMEM (ATCC® 30-2003™) + 25 mM HEPES (Gibco® 15630-080) + 0.2% BSA Fraction V (Gibco® 15260-037) + 2 µg/mL TPCK-treated Trypsin (USB® 22725)
Temperature
35°C
Atmosphere
95% Air, 5% CO2
Incubation
2-3 days at 35°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80% of the monolayer.
Recommendation for infection
For best results, infection should be performed on a 80-90% confluent, 18-48 hour old cellular monolayer. Prepare dilution of virus in minimum amount of volume (e.g. 1 mL per 25 cm2). Wash monolayer three times with PBS or serum free medium prior to inoculation. Adsorb virus dilution for 1-2 hours at 35°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Handling notes
This item was isolated in cell culture by three passages in MDCK cells.
Key abbreviations
BSA, Bovine serum albumin
CPE, Cytopathic effect
EMEM, Eagle's Minimum Essential Medium
HEPES, N-(2-Hydroxyethyl)piperazine-N'-(2-ethanesulfonic acid)
MDCK, Madin Darby canine kidney cells
PBS, Phosphate-buffered saline
TPCK, L-1-tosylamido-2-phenylethyl chloromethyl ketone
Quality control specifications
Verification method
Whole-genome Sequencing
History
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