Vaccinia virus 拉丁名
VR-2153 编号
Vaccinia virus strain vTF7-3 [Wr] is propagated in BS-C-1 cells (ATCC CCL-26). This strain was constructed in vitro by transfecting vaccinia virus-infected cells with pTF703 cells (ATCC 67202) and was deposited by the National Institutes of Health. It expresses bacteriophage T[7] RNA polymerase.
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Product categoryViruses
Product typeAnimal virus
ClassificationPoxviridae, OrthopoxvirusStrain designationvTF7-3 [Wr]Isolation sourceConstructed in vitroProduct formatFrozenStorage conditions-70°C or colder
Specific applications
Expresses bacteriophage T[7] RNA polymerase.
CommentsCells which have been infected with this construct can be transfected by plasmids which contain a gene targeted for expression inserted between a T7 promoter and T7 terminator.
Expresses bacteriophage T[7] RNA polymerase.
Recombinant virus constructed in vitro.
Expression of the T7 RNA polymerase is directed by the P7.5 promoter which includes early and late regulatory signals.
Constructed by transfecting vaccinia virus-infected cells with pTF703 (ATCC 67202).
Mycoplasma contaminationNot detectedTechnical informationATCC Technical Services does not have technical information on patent deposits that are not produced or characterized by ATCC. Additional information can be found in the corresponding patent available from the patent holder or with the U.S. and/or international patent office.
HostBS-C-1 (ATCC CCL-26)
Host Range: BSC-1, CV-1, HeLa, MRC-5BS-C-1 cells (ATCC CCL-26); CV-1 cells (ATCC CCL-70); HeLa cells (ATCC CCL-2); MRC-5 cells (ATCC CCL-171)
Effectscell death; cell rounding; CPE; plaque formation; stringing
Temperature 培养温度 37°C
Incubation2 - 3 daysHandling notesExpresses bacteriophage T(7) RNA polymerase.
Key abbreviationsBS-C-1, African green monkey kidney cells
CPE, Cytopathic effect
CV-1, African green monkey kidney cells
FBS, Fetal bovine serum
HeLa, Human cervical carcinoma cells
MRC-5, Human embryonic lung cells
NIH, National Institutes of Health
DepositorsNIH, B Moss, NIHPatent depositoryThis material was deposited with the ATCC Patent Depository to fulfill U.S. or international patent requirements. This material may not have been produced or characterized by ATCC. As an International Depository Authority (IDA) for patent deposits, ATCC is required to complete viability testing only at time of initial deposit of patent material. Patent deposits are made available on behalf of the Depositor when the pertinent U.S. or international patent is issued, but material may not be used to infringe the patent claims.
Patent number5,550,035