Bovine parainfluenza virus 3
VR-3377 ™

Bovine parainfluenza virus 3 strain SF-3 is propagated in BT cells (ATCC CRL-1390). This animal virus was isolated in 1958 from a calf showing signs of shipping fever in Maryland.
Product category
Viruses
Product type
Animal virus
Classification
Paramyxoviridae, Paramyxovirus
Strain designation
SF-3
Isolation source
Calf showing signs of shipping fever
Geographical isolation
United States; Maryland
Applications
Agricultural research
Infectious disease research
Respiratory disease research
Product format
Frozen
Storage conditions
-70°C or colder
Characteristics
Comments
ATCC VR-3377 was derived from ATCC VR-281.
Handling information
Host
BT (ATCC CRL-1390)
Effects
cell rounding; cell sloughing; cell detachment; cell clumping
Complete medium
DMEM (ATCC 30-2002) + 2% Horse serum
Temperature
37°C
Incubation
3-5 days at 37°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 90-99% of the monolayer.
Recommendation for infection
Plate cells 24-48 hours prior to infection and infect when cultures are 70-80% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1). Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere. End adsorption by adding virus growth medium.
Handling notes
ATCC VR-3377 was derived from ATCC VR-281.
Key abbreviations
°C, Degrees Celsius
CO2, Carbon dioxide
DMEM, Dulbecco's Modified Eagle's Medium
MOI, Multiplicity of infection
Quality control specifications
Volume
1.0 mL
History
Depositors
G. N. Frank and R. G. Marshall
Year of origin
1958
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