Influenza A virus (H1N1)
VR-1736™
Influenza A virus (H1N1) strain A/Virginia/ATCC1/2009 is propagated in MDCK (NBL-2) cells (ATCC CCL-34). This strain was isolated in 2009 from a nasopharyngeal specimen from a patient positive for Influenza A in Virginia. It can be used in respiratory disease and influenza research.
Product category
Viruses
Product type
Animal virus
Classification
Orthomyxoviridae, Influenzavirus A
Strain designation
A/Virginia/ATCC1/2009
Isolation source
Nasopharyngeal specimen from a patient positive for Flu A.
Geographical isolation
United States; Virginia
Applications
Infectious disease research
Respiratory disease research
Product format
Frozen
Storage conditions
-70°C or colder
General
Specific applications
Emerging infectious disease research
Respiratory research
Influenza Research
Characteristics
Comments
Item analyzed using Real-time RT-PCR to determine strain is of pandemic swine Influenza A origin.
Mycoplasma contamination
Not detected
Handling information
Host
MDCK (NBL-2) (ATCC CCL-34)
Effects
CPE; cell degeneration; cell rounding
Complete medium
EMEM (ATCC 30-2003) + 2 µg/mL TPCK treated Trypsin + 0.125% BSA Fraction V + 10 mM HEPES Buffer
Temperature
35°C
Atmosphere
95% Air, 5% CO2
Incubation
Incubate infected culture for 2 to 5 days at 35°C in a humidified 5% CO2 atmosphere, until CPE are well advanced through 90% of the culture.
Recommendation for infection
Plate cells 24 hours in advance and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus diluted with VGM to provide a MOI 0.1. Adsorb for 1 hour at 35°C in a humidified 5% CO2 atmosphere. End adsorption by adding virus growth medium.
Handling notes
Item analyzed using Real-time RT-PCR to determine strain is of pandemic swine Influenza A origin.
Key abbreviations
°C, Degrees Celsius
CO2, Carbon dioxide
CPE, Cytopathic effect
MOI, Multiplicity of infection
MDCK, Madin Darby canine kidney cells
EMEM, Eagle's Minimum Essential Medium
TPCK, L-1-tosylamido-2-phenylethyl chloromethyl ketone
BSA, Bovine serum albumin
HEPES, N-(2-Hydroxyethyl)piperazine-N'-(2-ethanesulfonic acid)
μg, Microgram
mL, Milliliter
mM, millimolar
History
Depositors
ATCC
Type of isolate
Human
Year of origin
2009