Human herpesvirus 3
VR-1832
Product category Viruses
Product type Animal virus
Classification Herpesviridae, Varicellovirus
Strain designation Oka
Common name Varicella zoster virus
Isolation source Vesicle from a 3-year-old male
Geographical isolation Japan
Applications Infectious disease research
Assay development
Vaccine development
Product format Frozen
Storage conditions
Vapor phase of liquid nitrogen
Virucide testing
In vitro infectivity assay
Antiviral Studies
Spiking studies
ELISpot Assay
Virus neutralization assay
Virus ultrastructural studies
Immunological-based assays
PCR-based assay validation
Research and development
Assay development
Vaccine development
Characteristics
Comments
Live, attenuated vaccine strain. Thermosensitive compared with other wild type strains. Depositor screened by Neurovirulence safety test, and result was compliant.
Handling information
Host
MRC-5 (ATCC CCL-171)
Effects CPE; cell rounding; cell degeneration
Complete medium EMEM (ATCC 30-2003) + 2% FBS (ATCC 30-2020)
Temperature 37°C
Atmosphere 95% Air, 5% CO2
Incubation
3-4 days at 37°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 60% of the monolayer.
Recommendation for infection
Plate cells 16-24 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.01). Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere. End adsorption by adding virus growth medium. Replace virus growth medium at one day post-inoculation to remove DMSO from the culture.
Harvest by removing culture medium and adding cell dissociation buffer. Dilute in freeze medium containing a final concentration of 7% DMSO and preserve with a controlled-rate freeze (1°C/minute).
Handling notes
Live, attenuated vaccine strain. Thermosensitive compared with other wild type strains. Depositor screened by Neurovirulence safety test, and result was compliant.
Key abbreviations
°C, Degrees Celsius
CO2, Carbon dioxide
DMSO, Dimethyl sulfoxide
EMEM, Eagle's Minimum Essential Medium
FBS, Fetal bovine serum
MOI, Multiplicity of infection
Quality control specifications
Volume
1.0 mL
History
Depositors
Y Oyabu, The Research Foundation for Microbial Diseases of Osaka University
Patient age
3 years
Patient gender
Male
Legal disclaimers
Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty
The product is provided 'AS IS' and the viability of ATCC products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid. Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.
Disclaimers
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.
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