Product category
Viruses
Product type
Animal virus
Classification
Paramyxoviridae, Rubulavirus, Human parainfluenza virus 4
Strain designation
M-25
Common name
HPIV-4a
Genome sequenced strain
Yes
Isolation source
Throat swab of patient with mild respiratory tract illness
Applications
Infectious disease research
Respiratory disease research
Product format
Frozen
Storage conditions
-70°C or colder
Comments
ATCC VR-3350 was derived by cell culture adaptation of ATCC VR-1378 in Vero.STAT1 KO (ATCC CCL-81-VHG). ATCC VR-3350 has not been compared for sequence or infectivity in primary cell culture and tissue culture.
Handling information
Host
Vero.STAT1 KO (ATCC CCL-81-VHG)
Effects
cell rounding; elongation (fibroblast-like morphology); cell sloughing
Complete medium
Eagle’s Minimum Essential Medium (ATCC 30-2003) + 10 BAEE/mL of TPCK-Trypsin
Temperature
36°C
Recommendation for infection
Plate cells 24-48 hours prior to infection and infect when cultures are 85-100% confluent. Remove medium and rinse the flask/plate twice with neat EMEM because the presence of serum will inactivate the TPCK-Trypsin. Inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1). Adsorb 1-2 hours at 36°C in a humidified 5% CO2 atmosphere on a rocker. End adsorption by adding virus growth medium.
Handling notes
ATCC VR-3350 was derived by cell culture adaptation of ATCC VR-1378 in Vero.STAT1 KO (ATCC CCL-81-VHG). ATCC VR-3350 has not been compared for sequence or infectivity in primary cell culture and tissue culture.
Key abbreviations
°C, Degrees Celsius
CO2, Carbon dioxide
EMEM, Eagle's Minimum Essential Medium
FBS, Fetal bovine serum
MOI, Multiplicity of infection
Quality control specifications
Verification method
Whole-genome Sequencing
History
Depositors
JC Hierholzer, Centers for Disease Control and Prevention
Type of isolate
Human
Legal disclaimers
Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty
The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid. Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.
Disclaimers
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