ATCC medium: 2091 Sulfobacillus disulfidooxidans medium
Solution A:
(NH4)2SO4.....................3.0 g
KCl..........................0.1 g
KH2PO4 .......................0.5 g
MgSO4 . 7H2O .................0.5 g
Yeast extract................0.1 g
Distilled water..............1.0 L
Solution B:
Glutathione..................2.5 g
Distilled water.............10.0 ml
Prepare Solution A and adjust pH to 2.25 with 0.1 N sulfuric acid. Autoclave at 121C for 20 minutes.
Filter-sterilize Solution B and add 1.0 ml to 99 ml of Solution A.
Final pH should be 2.5. Note: Original formula calls for Glutamic Acid. Requestor has specified Glutathione.