ATCC medium: 62 Escherichia medium
Trypticase Peptone (BD 211921).....6.0 g
K2HPO4 .............................0.2 g
FeSO4 . 7H2O .......................5.0 mg
MgSo4 . 7H2O .......................0.2 g
L-Asparagine.......................0.15 g
Glycerol...........................2.0 g
Vitamin B12........................0.4 mg
Agar (if needed)..................15.0 g
Distilled water....................1.0 L
Adjust medium for final pH 7.2. Autoclave at 121C for 15 minutes.