Quantitative Synthetic Human coronavirus NL63 RNA 拉丁名
(ATCC® VR-3263SD™) 统一编号
Agent Quantitative Synthetic Human coronavirus NL63 RNA
Applications ATCC® Genuine Nucleics can be used for assay development, verification, validation, monitoring of day-to-day test variation, and lot-to-lot performance of molecular-based assays. The quantitative format allows for the generation of a standard curve for quantitative PCR (qPCR) to determine viral load.
Biosafety Level 生物安全等级 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format 提供形式 frozen
Specification range: 1 x 105 to 1 x 106 copies/µL
100 µL per vial with Biomatrica RNAstable
Storage Conditions 保藏条件 -70°C or colder
Intended Use The synthetically engineered sequence of the product constitutes intellectual property belonging to ATCC. Unauthorized use, including sequencing, modification, or reverse-engineering, of the product is expressly prohibited without prior ATCC consent.
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Comments 注释
Manufactured under ISO 13485 guidance
Preparation includes fragments from NSP3 (ORF 1A), Rdrp (nsp12), NTPase (nsp13), nsp16, spike protein, nucleocapsid, and 3’ untranslated regions.
The following primers and probe can be used with this nucleic acid preparation:
Forward Primer (5’ to 3’): AGGACCTTAAATTCAGACAACGTTCT
Reverse Primer (5’ to 3’): GATTACGTTTGCGATTACCAAGACT
Probe (5’ to 3’): /56-FAM/TAACAGTTTTAGCACCTTCCTTAGCAACCCAAACA/36-TAMSp/
Name of Depositor ATCC
Special Collection RNA
References 参考文献
Cui LJ, et al. Human Coronaviruses HCoV-NL63 and HCoV-HKU1 in Hospitalized Children with Acute Respiratory Infections in Beijing, China. Adv Virol 2011: 129134, 2011. PubMed: 22315599