名称SW620/LUC(人结肠癌细胞(荧光素酶标记))(DMEM)(STR鉴定正确)
种属人类
年龄(性别)男性,51岁
组织来源结直肠腺癌,来自转移淋巴结
生长特性贴壁细胞
细胞形态上皮细胞样
生物安全等级1
生长培养基DMEM+10%FBS+1%P/S
推荐传代比例1:3-1:4
推荐换液频率2~3次/周
倍增时间~20-26小时
冻存条件
冻存液:55%基础培养基+40%FBS+5%DMSO
温度:液氮
培养条件
气相:空气,95%;CO2,5%
温度:37℃
致瘤性Yes,in nude mice(Tumors developed within 21 days at 100%frequency(5/5)in nude mice inoculated subcutaneously with 1×10^7 cells).
抗原表达情况Blood Type A;Rh+
基因表达情况Carcinoembryonic antigen(CEA)0.15 ng/10^6 cells/10 days;transforming growth factor alpha;matrilysin.The cells are negative for expression of CSAp(CSAp-)and colon antigen 3,negative.The cells are positive for keratin by immunoperoxidase staining.The line is positive for expression of c-myc,K-ras,H-ras,N-ras,Myb,sis and fos oncogenes.
保藏机构ATCC;CCL-227 ECACC;87051203